CRISPRCas9 produces anti hepatitis B virus effect in hepatoma cells and transgenic mouse
Wei Zhu , Kun Xie , Yuanjian Xu , Le Wang , Kaiming Chen , Longzhen Zhang , Jianmin Fang
[ABSTRACT] Chronic infection of hepatitis B virus (HBV) is at risk of liver cirrhosis and hepatocellular carcinoma and remains one of the major public health problems worldwide. It is a major barrier of persistence HBV cccDNA under current antiviral therapy as novel strategies of disrupting HBV cccDNA is pressing. The (CRISPR)/Cas9 system is presently emerging in gene editing and we also apply it for targeting and deleting the conserved regions of HBV genome ...
[Enzyme-linked immunosorbent assay (ELISA)] The cell cytotoxicitys of Huh7 and HepG2 after transfection with pCas9 or pX330 for 48 h were analyzed by LDH ELISA kit ( Jianglai, Shanghai, China [1]) for measuring supernanant LDH level. Treatment of 200 ng/mL LPS on Huh7 and HepG2 for 48 h was considered as the positive control. All values (cytotoxicity%) were calculated by the positive control value as the protocol described ...
[備注] [1]“江萊生物”10年以來一直致力于免疫學技術的積累與沉淀,目前江萊生物實驗室占地約3000平米,技術研發團隊50余人,配備了全球最先進的分析和檢測設備,我們通過自主創新以及與國內外的科研單位合作研發,不斷突破新技術,目前我們研發了上千種指標,質量成熟,并長期出口到歐美市場,贏得了眾多科研機構的認可 ...
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